Technology development for low cost and high-throughput sequencing of microRNAs and V(D)J recombination of the immune repertoire.
Francois Vigneault,
Post-Doctoral Fellow
Harvard Medical School
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Abstract: Although most miRNAs studies rely on the detection of previously reported miRNAs, the most powerful approach to identify and quantify expression of new miRNAs remains direct cloning and sequencing. To this extent, we have developed a procedure to facilitate miRNA capture by ligation by barcoding samples, thus allowing multiplexing and considerably minimizing the per-sample cost on next-generation sequencing platforms. We are now focused on the development of technology for in situ sequencing of microRNAs in tissue section as well as the development of an approach for sequencing the V(D)J recombination of the immune repertoire in a single individual which we termed the personal VDJ-ome
Presenter Biography: Dr. Francois Vigneault is currently a post-doctoral fellow in the laboratory of George Church. His focus is on developing innovative solution for low cost and high-throughput sequencing for various biological problems.
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